By: Organic Synthesis: Techniques and Strategies

Organic Synthesis: Techniques and Strategies — Wed, 29 May 2024 17:16:32 +0000

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By: Dániel Budai

Dániel Budai — Sat, 06 Apr 2024 21:34:09 +0000

There is also a chromatography technique which is actually older than HPLC but still relatively unknown despite it does not use a column, only solvents so scaling up from laboratory scale to industrial scale is almost linear. It is called Centrifugal Partition Chromatography (or CPC in short) and it uses a biphasic solvent system which can consist of multiple solvents. One of the two phases will be the stationary phase, and the other will be the moving phase (in this case deciding between the more dense or less dense phase to be the stationary one is like deciding between normal and reverse phase column). The stationary phase is immobilized by the spinning of a rotor (centrifugal force), so it will act similarly to a solid phase, while the mobile phase is percolated through it with high pressure. The drive of the separation is the difference between the partition coefficient of each compounds.

Comments on: Natural Product Isolation (2) – Purification Techniques, An Overview

By: Organic Synthesis: Techniques and Strategies

By: Dániel Budai